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DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml
Ideal for cell dissociation and other procedures requiring controlled ion conditions, ensuring consistent and reliable results.
DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml
Item #: 222174365

DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml

Item #: 222174365

FJD 74

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Ideal for cell dissociation and other procedures requiring controlled ion conditions, ensuring consistent and reliable results.
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Product Details

Shop DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml online at a best price in Fiji. B0F3773XRY
Brand NameCryoKing
ManufacturerBIOLOGIX
Part NumberDPBS-1000-1
Unit Count1.0 Count
Included ComponentsDPBS Buffer
BrandCryoKing
Item Weight2.2 lbs (1 kg)

Product Description

DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml

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Customer Questions & Answers

  • Question: What is DPBS Buffer 1X?

    Answer: DPBS Buffer 1X, or Dulbecco's Phosphate-Buffered Saline, is a balanced salt solution widely used in biological and laboratory applications. It provides essential ions and maintains physiological pH, which is crucial for cell culture, washing cells, or diluting biological samples. Without calcium and magnesium, this formulation is ideal for experiments where these ions might interfere, ensuring accurate results during sensitive experiments.
  • Question: How is DPBS Buffer 1X used in cell culture?

    Answer: DPBS Buffer 1X is extensively utilized in cell culture practices to rinse cells prior to trypsinization or to remove serum from the culture medium. This gentle solution helps maintain osmotic balance and prevents cell lysis during handling. Ideal for sensitive cell types, it ensures cells are treated with the utmost care while preparing for experiments or analysis.
  • Question: Is DPBS Buffer 1X sterile?

    Answer: Yes, the DPBS Buffer 1X is sterilized through a 0.1µm filter, ensuring that it is free from contaminants. The sterilization process is crucial in laboratory settings where maintaining aseptic conditions is necessary for reliable results. Using sterile DPBS minimizes the risk of microbial contamination during cell culture and other sensitive laboratory procedures.
  • Question: What is the significance of pH 7.4 in DPBS Buffer 1X?

    Answer: The pH of 7.4 in DPBS Buffer 1X is physiologically relevant and mimics the natural environment of human tissues. This pH level is critical for maintaining cellular functions and ensuring optimal reactions during experiments. Using a buffer that maintains this neutral pH is essential for any studies involving live cells or biological samples to reflect physiological conditions accurately.
  • Question: Can DPBS Buffer 1X be used for washing cells?

    Answer: Absolutely! DPBS Buffer 1X is often used for washing cells before further processing or analysis. By removing unwanted substances or leftover media, it prepares the cells for experiments such as flow cytometry, immunostaining, or any assay where cellular integrity is important. This washing step is vital to ensure that only the targeted cells and techniques are utilized, improving the accuracy of results.
  • Question: What is the difference between DPBS with and without calcium and magnesium?

    Answer: DPBS Buffer 1X without calcium and magnesium is specifically formulated for situations where these ions might interfere with protocols, such as cell detachment or trypsinization. By omitting these ions, researchers can prevent unwanted cellular interactions and ensure better cell viability post-experiment. This makes it an essential choice for researchers focusing on cellular assays or sensitive preparations.
  • Question: Is DPBS Buffer 1X suitable for in vitro experiments?

    Answer: Yes, DPBS Buffer 1X is highly appropriate for various in vitro experiments including cell culture, enzyme studies, and reconstitution of protein solutions. Its balanced ion composition and physiological pH make it optimal for maintaining cell health and promoting stable experimental conditions. Researchers use it extensively due to its versatility across different biological protocols.
  • Question: What kinds of experiments is DPBS Buffer 1X commonly used in?

    Answer: DPBS Buffer 1X finds application in experiments like cytotoxicity assays, immunofluorescence, and enzyme activity assays. Due to its gentle handling properties, it is favored for preparing and washing cells without compromising integrity. Labs utilize it for accurate biological assays, ensuring that cells are treated in a stable, physiologically relevant environment.
  • Question: How is DPBS Buffer 1X stored?

    Answer: To maintain its efficacy, DPBS Buffer 1X should be stored at 2-8°C in a sealed container to prevent contamination. Once opened, it’s advisable to use it within a suitable timeframe to ensure sterility. Proper storage is crucial in retaining the buffer's quality and reliability for experiments, providing consistent results across various applications.
  • Question: Where can I buy DPBS Buffer 1X, 1000ml in Fiji?

    Answer: You can purchase DPBS Buffer 1X, 1000ml from Ubuy in Fiji. Ubuy offers a variety of laboratory supplies, ensuring you have access to high-quality products conveniently. Their platform enables easy ordering and prompt service that caters to your laboratory needs, streamlining your purchasing process for essential research materials.

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